Download Nonisotopic DNA Pr...

Format: Paperback

Language: English

Pages: 358

Publisher: Academic Press; Spi edition (April 13, 1992)

ISBN: 0124262961

Format: PDF / Kindle / ePub

Size: 8 MB

Downloadable formats: PDF


lately many nonisotopic tools of probing particular DNA sequences were built as replacements for radioactive labels, equivalent to 32phosphorous and 125iodine. This e-book brings all of those new tools jointly in a single handy, simply available resource. It allows researchers to choose the nonisotopic approach most suitable to their program and to take advantage of it to greatest virtue by way of following the easy directions provided.This booklet comprises chapters on colorimetric, bioluminescent, chemiluminescent, fluorescent, and time-resolved fluorescent detection tools. every one bankruptcy has been written by means of the inventor or developer of a specific nonisotopic procedure and hence offers a professional account of the tactic. each one chapterpresents important historical past info and specified, step by step, easy-to-follow, experimental methods for labeling and detection. provides broad functional informationCovers significant different types of nonisotopic labels and proceduresPresents history details for every methodProvides suggestions and designated experimental methods for labeling and detecting DNA sequences byFluorescenceChemiluminescenceBioluminescenceColorimetry

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